Q:10185(Mouse Tissue Direct PCR Kit With Dye)和 10181(Mouse Tissue Direct PCR Kit)样本裂解温度和裂解操作一样吗?
A:均不一样。样本裂解温度区别: 10185:95℃,10-30min。10181:65℃,10-30 min。
裂解操作区别:10185:裂解组分A 和终止裂解组分 B 分开加,终止裂解混匀即可。 10181:裂解组分A 和终止裂解组分B 一起加,但终止裂解需要 5min。
Q:小鼠组织直接pcr 是鼠趾还是鼠尾好?需不需要纯化?
A:不需要纯化,离心取上清(选做),没比较鼠尾还是鼠趾效果,一般都是用鼠尾
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